Application of purine and urea types of cytokinins inshoot multiplication of Anise (Pimpinella anisum L.) invitroPimpinella anisum L. or sweet anise is an annual–herbaceous plantbelongs to the Umbelliferae family. The fruit of anise contains of essentialoil, which is mainly consisted of trans-anethol (90%). Essential oils ofanise is mainly used as an antiseptic, antispasmodic, anticancer,carminative, expectorant and has also been used as component in soap,perfumery, tooth paste, and skin cream productions. Since this crop ismainly cultivated in sub tropical region, anise cultivation in Indonesia hasnot been performed. To obtain sufficient numbers of anise plantingmaterials in vitro propagation was conducted by applying benzyl aminopurine (BAP) and thidiazuron (TDZ). In this research TDZ or BAP wereapplied at various concentrations (0,1 mg/l: 0.2 mg/l; 0.3 mg/l; 1 mg/l; 2mg/l; 3 mg/l), to induce shoots in MS-solid culture media. The highestnumber of shoots obtained in those two type of cytokinins containingmedia from the initiation stage were subcultured into the mediasupplemented with different cytokinins (TDZ to BAP or BAP to TDZ) at0.3 mg/l or 3 mg/l levels. The results showed that medium with theaddition of TDZ resulted in higher numbers of shoot (3,26-6,28) than thatof medium with an addition of BAP (1,86-2,78). However, rosette shootswere dominant in TDZ containing medium. On the other hand, mediumwith an addition of BAP resulted in less numbers of shoots with tallernodes. Subculture of anise into different kinds of cytokinins increased thenumbers of proliferated-shoots and recovered the abnormal shoots.
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